Home > Sandwich ELISA Kits > Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α;CCL3) ELISA Kit

Product Name:

Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α;CCL3) ELISA Kit

Cat.#:

JL11885

Regulatory Status:

RUO

Alternative:

CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.62-1000pg/mL

Sensitivity:

5.99pg/mL

Specificity:

This kit recognizes Human MIP-1α;CCL3 in samples. No significant cross-reactivity or interference between Human MIP-1α;CCL3 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MIP-1α;CCL3

Chemokine (C-C motif) ligand 3 (CCL3) also known as macrophage inflammatory protein 1-alpha (MIP-1-alpha) is a protein that is encoded by the CCL3 gene.CCL3 is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes through binding to the receptors CCR1, CCR4 and CCR5.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	1000	500	250	125	62.5	31.25	15.62	0
OD	2.07	1.33	0.87	0.58	0.4	0.27	0.18	0.08
Corrected OD	1.99	1.25	0.79	0.5	0.32	0.19	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MIP-1α;CCL3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	98
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human MIP-1α;CCL3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	86-96	90-110
1 : 2	Average (%)	91	91	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108